고성능 액체 크로마토그래피를 이용한 마황 및 월비가출탕 전탕액에서 에페드린류의 동시분석
Stimultaneous Determination of Ephedrine Alkaloids in Ephedra sinica and Wolbigachul-tang by High Performance Liquid Chromatography
송미영⋅김정옥1
⋅임현희1
⋅김호준2
Miyoung Song, Jung-Ok Kim1, HyunHee Leem1, Hojun Kim2
더리셋한의원, 1한국한의학진흥원, 2동국대학교 한의과대학 한방재활의학교실
The Reset Korean Medicine Clinic, 1National Institute for Korean Medicine Development, 2Department of Rehabilitation Medicine of Korean Medicine, Dongguk University
Hojun Kim
Department of Rehabilitation Medicine of Korean Medicine, Dongguk University Ilsan Oriental Hospital, 27, Dongguk-ro, Ilsandong-gu, Goyang 10326, Korea
Tel: +82-31-961-9111
Fax: +82-31-961-9009
E-mail: kimklar@dongguk.ac.kr
Department of Rehabilitation Medicine of Korean Medicine, Dongguk University Ilsan Oriental Hospital, 27, Dongguk-ro, Ilsandong-gu, Goyang 10326, Korea
Tel: +82-31-961-9111
Fax: +82-31-961-9009
E-mail: kimklar@dongguk.ac.kr
Received November 6, 2020; Revised December 12, 2020; Accepted December 14, 2020.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.- Abstract
- Objectives: Ephedra sinica and Wolbigachul-tang which contains Ephedra sinica are used to treat obesity in Korean medicine. The aim of this study was to analyze the quantities of ephedrine alkaloids by high-performance liquid chromatography.
Methods: The analysis was performed using a YMC-Triat C18 column with operating at 25°, and UV detection at 210 nm. The mobile phase used a gradient flow with 0.1% H3PO4 in water and acetonitrile. Specificity, linearity, precision, accuracy, limit of detection, and limit of quantification were measured for validation anaylsis. This method was applied to analyze the quantities of ephedrine alkaloids in Ephedra sinica and Wolbigachul-tang.
Results: The concentration per Ephedra sinica (gram) of ephedrine and pseudoephedrine in Ephedra sinica decoction are 4.74±0.22 mg and 2.19±0.10 mg, respectively and in Wolbigachul-tang decoction are 6.39±0.34 mg and 2.97±0.21 mg, respectively. The retention time of ephedrine was 23.6 min and that of pseudoephedrine was 25.8 min, and norephedrine and methylephedrine were not detected.
Conclusions: In conclusion, analyzed the concentration of ephedrine alkaloids in Ephedra sinica and Wolbigachul-tang by the developed validation method. - Keywords : Ephedra sinica, Wolbigachul-tang, Ephedrine, Pseudoephedrine, High pressure liquid chromatography
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