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65종 한약처방이 3T3-L1 지방전구세포의 지방 분화에 미치는 효능 비교 연구
Comparative Study on the Differentiation Effect of Adipogenesis in 3T3-L1 Preadipocyte by 65 Herbal Medicine Prescriptions
J Korean Med Obes Res 2020;20:78-87
Published online December 31, 2020;  https://doi.org/10.15429/jkomor.2020.20.2.78
Copyright © 2020 The Society of Korean Medicine for Obesity Research.

최혜민⋅유병우⋅김민주⋅김정옥
Hye-Min Choi, Byung-Woo Yu, Min-Ju Kim, Jung-Ok Kim

한국한의약진흥원 한의기술 R&D 2팀

Korean Medicine R&D Team 2, National Institute for Korean Medicine Development
Jung-Ok Kim
Korean Medicine R&D Team 2, National Institute for Korean Medicine Development, 94 Hwarang-ro, Gyeongsan 38540, Korea
Tel: +82-53-810-0395
Fax: +82-53-810-0245
E-mail : kjo1226@nikom.or.kr
Received October 30, 2020; Revised November 27, 2020; Accepted December 9, 2020.
cc This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Objectives: To expand and provide information on the efficacy of herbal medicines, anti-obesity effects were evaluated. In many studies, plant-derived components with anti-obesity efficacies have been investigated for their potential inhibitory effects on 3T3-L1 preadipocyte cells. The purpose of this study was to investigate the anti-obesity effects of 65 herbal medicine in 3T3-L1 preadipocyte cells.
Methods: Preferentially, 3T3-L1 cells were treated with 65 herbal medicines (500 µg/mL) during differentiation for 8 days. Next, 3T3-L1 cells were treated with selected herbal medicines at concentrations ranging from 50 to 200 µg/mL during differentiation for 8 days. The accumulation of lipid droplets was determined by Oil Red O staining. The expressions of genes related to adipogenesis were measured by reverse transcription polymerase chain reaction and Western blot analyses.
Results: Among the 65 kinds of herbal medicines, 13 herbal medicines that been shown to be effective against the accumulation of lipid droplets were selected. Finally, selected Banhasasim-tang and Samhwangsasim-tang showed inhibitory activity on adipocyte differentiation at 3T3-L1 preadipocytes without affecting cell toxicity. In addition, Banhasasim-tang and Samhwangsasim-tang significantly reduced the expression levels of several adipocyte marker genes including peroxisome proliferator activated receptor-γ and CCAAT/enhancer binding protein-α.
Conclusions: These results suggest that the ability of Banhasasim-tang and Samhwangsasimtang has inhibited overall adipogenesis and lipid accumulation in the 3T3-L1 cells. Banhasasim-tang and Samhwangsasim-tang may be a promising medicine for the treatment of obesity and related metabolic disorders.
Keywords : Herbal medicine, obesity, 3T3-L1


December 2020, 20 (2)
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