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당귀수산, 생강, 커큐민의 대사성 질환과 관련된 일과성 수용체 전압 이온통로조절에 관한 연구
Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels
J Korean Med Obes Res 2018;18:10-8
Published online June 30, 2018;  https://doi.org/10.15429/jkomor.2018.18.1.10
Copyright © 2018 The Society of Korean Medicine for Obesity Research.

김병주
Byung Joo Kim

부산대학교 한의학전문대학원 양생기능의학부

Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University
Byung Joo Kim
Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University, 49 Busandaehak-ro, Mulgeum-eup, Yangsan 50612, Korea
Tel: +82-51-510-8469
Fax: +82-51-510-8420
E-mail: vision@pusan.ac.kr
Received April 17, 2018; Revised May 17, 2018; Accepted May 31, 2018.
cc This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Objectives: Metabolic syndrome is correlated with increased cardiovascular risk and characterized by several factors, including visceral obesity, hypertension, insulin resistance, and dyslipidemia. Several members of a large family of nonselective cation entry channels, e.g., transient receptor potential (TRP) melastatin 7 (TRPM7) channels have been associated with the development of cardiovascular diseases. The purpose of this study was to investigate the effects of Dangkwisoo-san, ginger and curcumin on TRPM7 channel.
Methods: Human embryonic kidney (HEK) 293 cells stably transfected with the TRPM7 expression vectors were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 5 μg/mL blasticidin, and 0.4 mg/mL zeocin in a humidified 20% O2/10% CO2 atmosphere at 37℃. Whole-cell patch clamp recordings were obtained using an Axopatch 700B amplifier and pClamp v.10.4 software, and signals were digitalized at 5 kHz using Digidata 1422A.
Results: Dangkwisoo-san extract (100, 200, 300, 400, and 500 μg/mL) inhibited the outward and inward TRPM7 whole-cell currents at dose dependent manner and the half maximal inhibitory concentration (IC)50 of Dangkwisoo-san was 218.3 μg/mL. Also, ginger extract (100, 200, 300, 400, and 500 μg/mL) inhibited the outward and inward of TRPM7 whole-cell currents in a dose dependent manner and the IC50 of ginger was 877.2 μg/mL. However, curcumin had no effects on TRPM7 whole-cell currents.
Conclusions: These results suggest that both Dangkwisoo-san and ginger have good roles to inhibit the TRPM7 channel, suggesting that Dangkwisoo-san and ginger are considered one of the candidate agents for the treatment of metabolic syndrome such as cardiovascular disease.
Keywords : Metabolic syndrome, Ginger, Curcumin


June 2018, 18 (1)
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