Fig. 5. Effect of COME treatment on the expression of adipogenesis- and lipolysis-related genes in the epididymal adipose tissues of high-fat diet-fed C57BL/6N mice. Mice fed with high-fat diet were treated with COME or GCE by oral gavage for 8 weeks. The total RNA in epididymal adipose tissue was isolated and reverse transcribed, and a real-time PCR was conducted. The expression of (A) FAS, (B) aP2, (C) CPT-1, and (D) HSL mRNA was normalized to the expression of GAPDH mRNA and presented relative to the CD group. Values are expressed as the mean±SEM (n=8). COME: Cydonia oblonga Miller fruit extract, GCE: Garcinia cambogia extract, CD: control diet, HFD: high-fat diet, H+C: HFD+400 mg/kg body weight/day Cydonia oblonga Miller fruit extract, H+G: HFD+400 mg/kg body weight/day Garcinia cambogia extract, FAS: fatty acid synthase, aP2: adipocyte protein 2, CPT-1: carnitine palmitoyl transferase-1, HSL: hormone-sensitive lipase, GAPDH: glyceraldehyde 3-phosphate dehydrogenase, SEM: standard error of the mean. Means without a common letter differ at P<0.05.
