Fig. 4. Effect of COME treatment on the expression of adipogenic transcription factors in the epididymal adipose tissues of high-fat diet-fed C57BL/6 mice. Mice fed with high-fat diet were treated with COME or GCE by oral gavage for 8 weeks. The total RNA in epididymal adipose tissue was isolated and reverse transcribed, and a real-time PCR was conducted. The expression of ( A) C /E BPα, ( B) P PARγ, and (C) SRE BP-1c mRNA w as normalized to the expression of GAPDH mRNA and presented relative to the CD group. Values are expressed as the mean±SEM (n=8). COME: Cydonia oblonga Miller fruit extract, GCE: Garcinia cambogia extract, CD: control diet, HFD: high-fat diet, H+C: HFD+400 mg/kg body weight/day Cydonia oblonga Miller fruit extract, H+G: HFD+400 mg/kg body weight/day Garcinia cambogia extract, C/EBPα: CCAAT/enhancer binding protein α, PPARγ: peroxisome proliferator-activated receptor γ, SREBP-1c: sterol-regulatory element-binding protein-1c, GAPDH: glyceraldehyde 3-phosphate dehydrogenase, SEM: standard error of the mean. Means without a common letter differ at P<0.05.
