Primer Sequences Used in This Study
| Target gene | Forward primer (5’-3’) | Reverse primer (5’-3’) |
|---|---|---|
| aP2 | GGATTTGGTCACCATCCGGT | TTCACCTTCCTGTCGTCTGC |
| C/EBPα | TGGACAAGAACAGCAACGAGTAC | GCAGTTGCCCATGGCCTTGAC |
| CPT-1 | CCTGGAAGAAACGCCTGATT | CAGGGTTTGGCGAAAGAAGA |
| FAS | AGGGGTCGACCTGGTCCTCA | GCCATGCCCAGAGGGTGGTT |
| HSL | CCGTTCCTGCAGACTCTCTC | CCACGCAACTCTGGGTCTAT |
| PPARγ | CAAAACACCAGTGTGAATTA | ACCATGGTAATTTCTTGTGA |
| SREBP-1c | CACTTCTGGAGACATCGCAAAC | ATGGTAGACAACAGCCGCATC |
| GAPDH | TGGGTGTGAACCATGAGAAG | GCTAAGCAGTTGGTGGTGC |
aP2: adipocyte protein 2, C/EBPα: CCAAT/enhancer binding protein α, CPT-1: carnitine palmitoyl transferase-1, FAS: fatty acid synthase, HSL: hormone-sensitive lipase, PPARγ: peroxisome proliferator-activated receptor γ, SREBP-1c: sterol-regulatory element-binding protein-1c, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
