Fig. 6. Effects of EH-CS combinations on adipogenesis-related proteins in FFAs-treated HepG2 cells. The phosphorylation levels of AMPK and ACC and SREBP1, PPARγ, C/EBPα and CPT1α protein expression levels in FFAs-treated HepG2 cells were immunoblotted and analyzed. HepG2 cells were treated with FFAs (1 mM) for 24 h and followed by treatment of EH-CS extracts for 48 h before protein extraction. EH: Ephedrae herba, CS: Coicis semen, FFAs: free fatty acids, AMPK: AMP-activated protein kinase, ACC: acetyl-CoA carboxylase, SREBP1: sterol regulatory element-binding protein 1, PPARγ: peroxisome proliferator-activated receptor gamma, C/EBPα: CCAAT enhancer binding protein alpha, CPT1α: carnitine palmitoyltransferase 1 alpha.