Fig. 3. Effects of EH-CS combination on HepG2 cells. (A) Effect of EH-CS combinations on HepG2 cell viability. HepG2 cells were incubated with different concentration of 5 ratios of EH-CS extracts (0-1,000 μg/ml) for 24 h. (B) Effect of EH-CS combinations on lipid accumulation in FFAs-treated HepG2 cells determined with Oil Red O staining. HepG2 cells were co-treated with FFAs (1 mM) and EH-CS extracts for 48 h. Results are the means±SDs of three independent experiments. (C) Microscopic images of ORO-stained HepG2 cells by treatment EH-CS extracts (x200). EH: Ephedrae herba, CS: Coicis semen, FFAs: free fatty acids, SD: standard deviation, ORO: Oil-Red O, NC: negative control, IND: FFAs-induced control. **P<0.01 vs. FFAs-untreated controls and ††P<0.01 vs. FFAs-treated control.
