Fig. 5. Effect of Cannabis root or stem extract on phosphorylated protein levels of HepG2 cells. (A) Representative immunoblot images of key markers of AMPK and AKT with its phosphorylated form. Metformin was used as a reference drug (PC). (B) Assessment of phosphorylated protein ratio by densitometric analysis of band intensities. Results of phosphorylated protein levels were divided by non-phosphorylated protein levels and normalized to the non- treated control. The results presented here are based on a series of experiments conducted independently at least three times. AMPK: AMP-activated protein kinase, AKT: protein kinase B. *P<0.05, **P<0.01 vs non-treated control group.
