Fig. 4. Effects of noni fruit juice (NFJ) on the interleukin (IL)-1β-induced inducible nitric oxide synthase (iNOS) expression in 3T3-L1 preadipocytes. (A) 3T3-L1 cells were incubated without or with IL-1β in the absence or presence of NFJ at the designated doses for 4 hours. Total cellular RNA from the conditioned cells was extracted and analyzed by reverse transcriptase polymerase chain reaction for measurement of the mRNA expression levels of iNOS or actin. (B) 3T3-L1 cells were incubated without or with IL-1β in the absence or presence of NFJ at the designated doses for 4 hours, followed measurement of cell viability by a MTS assay. Values are mean±standard error of results from three independent experiments with triplicate.
