Fig. 2. Effects of noni fruit juice (NFJ) at different concentrations on lipid accumulation, triglyceride (TG) content, and cell viability in differentiating 3T3-L1 preadipocytes. (A, B) To induce adipocyte differentiation, 3T3-L1 preadipocytes were grown in induction medium containing MDI, insulin, and fetal bovine serum for 8 days with or without of NFJ at the selected concentrations. On day 8 (D8), cell viability was calculated using MTS assay (A) and the intracellular lipid accumulation was estimated by Oil Red O staining (B) (x100). Values are mean±standard error (SE) of data from three independent experiments with triplicate. (C) Measurement of TG content in 3T3-L1 adipocytes (D8) that were grown in the absence or presence of NFJ at the designated concentrations by AdipoRed assay. Each experiment was conducted independently in triplication. Results are mean±SE of three independent experiments.
