Fig. 1. Effects of different inducers on lipid accumulation in differentiating 3T3-L1 preadipocytes. (A) To induce adipocyte differentiation, 3T3-L1 preadipocytes were grown in induction medium containing MDI, insulin, and fetal bovine serum (FBS) for 8 days. On day 8 (D8), the intracellular lipid droplets were evaluated by Oil Red O staining (upper panels in A). Phasecontrast cell images of the differentiation D8 were also taken (lower panels in A) (x100). (B) 3T3-L1 preadipocytes were induced to differentiate with an induction medium containing MDI, insulin, and FBS for 8 days. On day 8 (D8), intracellular triglyceride (TG) stores was assessed by AdipoRed assay.
