Fig. 5. Effects of AFB extract on muscle weights and the expression of energy metabolism-regulating proteins in gastrocneminus tissues of HFD-induced obesity mice. (A) The weights of gasctrocnemius tissues of mice were mearued at 12 weeks. The expression of p-IRS-1, IRS-1 (B), p-AKT, AKT (C), PI3K (D), p-AMPK, AMPK (E), GLUT4 (F), Sirt1 (G), and β-actin was determined by Western blot, respectively. β-actin was used an internal control. Histograms (A-G) were analyzed the expression of target protein per each total form or β-actin. Data were presented as mean±standard deviation (n=5 per a group). *P<0.05, **P<0.01, and ***P<0.001 vs. Nor: normal group, HFD: high fat diet-induced obesity control group, AFB: Allii Fistulosi Bulbus extract (500 mg/kg)-administrated group, Met: metformin (500 mg/kg)-administrated group, IRS-1: insulin receptor substrate 1, AKT: Ser/Thr kinase, PI3K: phosphoinositide 3-kinase, AMPK: adenosine monophosphate-activated protein kinase, GLUT4: Glucose transporter type 4, Sirt1: sirtuin1.
