Fig. 4. Effect of GHH on protein expression of AMPK and pAMPK in C2C12 myotubes by western blot. Differentiated cells were treated with metformin (0.75 mM), GHH (1 mg/ml). Data for bar graph are represented as mean±standard deviation using Kruskal- wallistest and Dunn’s post-hoc test. All experiments were repeated twice. *P<0.05 compared with the NC. ‘ns’ denotes no significant change. NC: normal control, Met: metformin treatment, GHH: Galgunhwanggumhwangryun-tang treatment, AMPK: adenosine monophosphate-activated protein kinase, pAMPK: phosphorylation AMPK.
