Primer Sequences for Quantitative Real Time Polymerase Chain Reaction
| Target | Forward | Reverse |
|---|---|---|
| Slc2a4 (known as GLUT4) | GGAAGGAAAAGGGCTATGCTG | TGAGGAACCGTCCAAGAATGA |
| Gk | TCCCTGTAAGGCACGAAGACAT | ATTGCCACCATCCATCTCA |
| Cpt1a | CTCAGTGGGAGCGACTCTTCA | GGCCTCTGTGGTACACGACAA |
| Nrf1 | CCCCCGAGGACACTTCTTATGATG | GGCCGTTTCCGTTTCTTCCCTGTT |
| Ppargc1a | AGCCGTGACCACTGACAACGAG | GCTGCATGGTTCTGAGTGCTAAG |
| Tfam | CACCCAGATGCAAAACTTTCAG | CTGCTCTTTATACTTGCTCACAG |
| Gadph | GACATCAAGAAGGTGGTGAAGCAG | ATACCAGGAAATGAGCTTGACAAA |
Slc2a4: glucose transporter type 4, Gk: glucokinase, Cpt1a: carnitine palmitoyltransferase IA, Nrf1: nuclear respiratory factors 1, Tfam: mitochondrial transcription factor A, Ppargc1a: peroxisome proliferator-activated receptor γ coactivator 1α, Gadph: glyceraldehyde 3-phosphate dehydrogenase.
