Fig. 2. Effect of GHH on glucose uptake in C2C12 myotubes. Differentiated cells were treated with metformin (0.75 mM), GHH (1 mg/ml). (a) glucose uptake was measured by the amount of 2-NBDG taken up by cells in fluorescence microscopy (×200). (b) Relative 2-NBDG uptake fluorescence intensity. Data for bar graph are represented as mean±standard deviation using Kruskal-Wallis test and Dunn’s post-hoc test. All experiments were repeated twice. *P<0.05 compared with the NC. ‘ns’ denotes no significant change. NC: normal control, Met: metformin treatment, GHH: Galgunhwanggumhwangryun-tang treatment, 2-NBDG: 2-[N-(7-160 nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose.
