Fig. 4. Effects of JN at 25 μl/ml on protein and mRNA expression levels of FAS and leptin in differentiating 3T3-L1 cells. (A, B) 3T3-L1 preadipocytes were induced to differentiate with an induction medium containing MDI, insulin, and FBS in the presence or absence of JN at 25 μl/ml, and harvested at D2, D5, and D8, respectively. Whole-cell lysates and total cellular mRNA at the indicated time point were extracted and analysed by Western blot (A) and RT-PCR (B) analysis, respectively. JN: Juknyeok, mRNA: messenger RNA, FAS: fatty acid synthase, MDI: 0.5 mM IBMX, 0.5 μM dexamethasone, and 5 μg/mL insulin, FBS: fetal bovine serum, D: day, RT-PCR: reverse transcription polymerase chain reaction.
