Fig. 2. Effects of JN at different concentrations on intracellular TG content and cell growth in differentiating 3T3-L1 cells. (A, B) 3T3-L1 preadipocytes were induced to differentiate with an induction medium containing MDI, insulin, and FBS in the presence or absence of JN at the indicated concentrations for 8 days. On D8, intracellular TG content was measured by AdipoRed assay (A). Values are mean±standard error of data from three independent experiments with triplicate. *P<0.05 vs control. On D8, the control or JN-treated 3T3-L1 cells, which cannot be stained with trypan blue dye, were counted under a microscope (B). The cell count assay was done in triplicates. Data are mean±SE of three independent experiments. *P<0.05 vs control. JN: Juknyeok, TG: triglyceride, MDI: 0.5 mM IBMX, 0.5 μM dexamethasone, and 5 μg/mL insulin, FBS: fetal bovine serum, D: day, LD: lipid droplets.
