Fig. 1. Effects of JN at different concentrations on intracellular lipid accumulation in differentiating 3T3-L1 cells. (A) The experimental scheme of 3T3-L1 preadipocytes differentiation. (B) 3T3-L1 preadipocytes were induced to differentiate with an induction medium containing MDI, insulin, and FBS in the presence or absence of JN at the indicated concentrations for 8 days. On D8, the intracellular LDs were assessed by Oil Red O staining (upper panels in [B]). Phase-contrast images of the control or JN-treated cells on D8 were also taken (lower panels in [B]). JN: Juknyeok, MDI: 0.5 mM IBMX, 0.5 μM dexamethasone, and 5 μg/mL insulin, FBS: fetal bovine serum, D: day, LD: lipid droplets.
