Fig. 4. Effect of R. sceleratus on the expression of AMPK/SREBP-1 signaling pathway in 3T3-L1 cells. 3T3-L1 preadipocytes were treated with various concentrations (50~200 μg/ml) of R. sceleratus. GAPDH was used as a loading control. The Data were presented as mean±standard error of the mean from three independent experiments. AMPK: AMP-activated protein kinase, p-AMPK: phosphorylated AMPK, SREBP-1: sterol regulatory element-binding protein-1, GAPDH: glyceraldehyde-3-phosphate dehydrogenase, C (-): control (-), C (+): control (+). ##P<0.01 compared with the control (-); *P<0.05,**P<0.01 compared with the control (+).
