Fig. 2. Effect of R. sceleratus extract on the differentiation of 3T3-L1 adipocytes via Oil Red O staining. 3T3-L1 preadipocytes were treated with various concentrations (50~200 μg/ml) of R. sceleratus. (A) Cell culture and differentiation protocol. (B) Intercellular lipid droplets were stained with Oil Red O. After 10 days differentiation, representative phasecontrast photomicrographs at 200x and 400x magnification depictied of 3T3-L1 adipocyte. Control (-) include a and b, control (+) include b and g, 50 μg/ml, include c and h, 100 μg/ml include d and i, 200 μg/ml include e and j. (C) Quantification of lipid accumulation by eluting with isopropanol. The Data were presented as mean±standard error of the mean from three independent experiments. Con (-): control without MDI media, Con (+): control with MDI media, MDI: 0.5 mM IBMX, 1 μM DEX, 10 μg/ml insulin, R. sceleratus: Ranunculus sceleratus, IBMX: 3-isobutyl-1-methylxanthine, DEX: dexamethasone, FBS: fetal bovine serum. #P<0.05 compared with the control (-); *P<0.05, **P<0.01 compared with the control (+).
