Journal of Korean Medicine for Obesity Research : eISSN 2288-1522 / pISSN 1976-9334

Table. 5.

Table. 5.

Characteristics of Animal Study

No. Author (yr) Characteristics of study Animal subjects (age) Treatment Experimental group (n) Duration of Treatment Outcomes
Disease model (induced)
1 Lee JH 53) (2011) Single dose Toxicity study ICR mice (5 weeks) Ohyaksungi-san (Wuyaoshunqi-san) Oral administration Control 0 mg/kg (10)1,250 mg/kg (10)2,500 mg/kg (10)5,000 mg/kg (10) Once Mortality, LD50, clinical signs, BW, organ weight, necropsy findings, hematologic values (WBC, RBC, HGB, HCT, MCHC, MCH, MCV, PLT)
-
2 Song MY 54) (2013) Efficacy pharmacology; anti-obesity SD rat (8 weeks) Fermented Samjung-hwan (SJH)by Lactobacillus plantarum (LP), Leuconostoc mesenteroides (LM), Bifidobacterium longum (BL)Oral administration Normal (6)Control (HFD) (6)HFD+unfermented SJH 200 mg/kg (6)HFD+LP fermented SJH 200 mg/kg (6)HFD+LM fermented SJH 200 mg/kg (6)HFD+BL fermented SJH 200 mg/kg (6) 8 weeks BW, TC, HDL, blood glucose
Obesity (HFD induced)
3 Yoo JS 55) (2015) Efficacy pharmacology; anti-obesity C57BL/6N mice (8 weeks) Herbal medicine Gangji-hwan (DF)-1, 2, 3, 4, Combination of Gangji-hwan-1 and Gamisoche-hwan (GSH)Oral administration Normal (9)Control (9)DF-1 (9)DF-2 (9)DF-3 (9)DF-1 (9)DF-1+GSH (9) 8 weeks BW, FER%, leptin, adioponectin, TG, FFA, glucose, insulin, -
Obesity (HFD induced)
4 Shin YR 56) (2015) Efficacy pharmacology; lipid metabolism C57BL/6 mice (6 weeks) Schizandrae fructus chloroform fraction (SFCF)Oral administration Normal (8)Control-HFD (8)HFD+SFCF (8) 3 weeks BW, food intake, water intake, TC, HDL, TG, AST, ALT, FBS, lipid accumulation in liver tissue, gen expression in liver tissue
Dyslipidemia (HFD induced)
5 Keum SO 57) (2015) Efficacy pharmacology; anti-obesity C57BL/6 mice (6 weeks) Gami-Cheongpyesagan-tang (GCST)Oral administration Normal (5)HFD (5)HFD+olistat (5)HFD＋GCST 100 mg/kg (5)HFD＋GCST 200 mg/kg (5)HFD＋GCST 400 mg/kg (5) 7 weeks Body weight, food intake, serological markers (total cholesterol, triglyceride, lipid contents, leptin, adiponectin and glutamic oxaloacetic transaminase/glutamic pyruvic transaminase) mRNA expression of obese-associating genes (SREBP-1c, FAS, SCD-1, ACO, CPT-1α)
Obesity (HFD induced)
6 Kim YM 58) (2015) Efficacy pharmacology; anti-obesity C57BL/6 mice (5 weeks) Agastache rugosa (AR)Oral administration Normal (5)Control-HFD (5)HFD+AR (5) 4 weeks Body weight, food intake, food efficiency, adipose tissue weight, histological changes (adipose tissue, liver), PPAR-γ, leptin
Obesity (HFD induced)
7 Kim SM 59) (2015) Efficacy pharmacology; reproductive organ damage SD rat (6 weeks) Palmijihwang-whan (PMJHW)Oral administration Intact (8)PTU (8)LT4 0.5 mg/kg (8)PMJHW 500 mg/kg (8)PMJHW 250 mg/kg (8)PMJHW 125 mg/kg (8) 4 weeks BW, organ weight (thyroid gland, testis, epididymis, prostate), TSH, T3, thyroxine, testosterone, DHT, FSH, testicular antioxidant defense systems (MDA, H2O2, SOD, catalase) histomorphometry (thyroid gland, testis, epididymis, prostate)
Hypothyroidism related rat male reproductive organ damages (Propylthiouracil induced)
8 Lee MC 60) (2015) Efficacy pharmacology; GI mobility ICR mice (8 weeks) Shengmai-san, Pyungwi-san Oral administration Normal (-)Control-negative (-) Poncirus trifoliata Raf. (-) Shengmai-san 0.01 g/kg (-) shengmai-san 0.1 g/kg (-) shengmai-san 1 g/kg (-) Pyungwi-san 0.01 g/kg (-) Pyungwi-san 0.1 g/kg (-) Pyungwi-san 1 g/kg (-) Once ITR
Normal Peritoneal irritation (acetic acid injection induced)Diabetes (STZ administration induced)
9 Song MY 61) (2016) General characteristics of obesity model induced by HFD C57BL/6 mice (5 weeks) - Normal (10)HFD (10) 8-16 weeks BW, FBS, OGTT, ITT, serum insulin, serum glucose, TC, TG, organ weight (liver, pancrease, epididymal fat), histological changes of liver tissue, basal body temperature, MW to BW
Obesity (HFD induced)
10 Song MY 62) (2016) Efficacy pharmacology; anti-obesity C57BL/6 mice (5 weeks) Coix lacrymajobi var. mayuen (Coix)Oral administration Normal (10)HFD (10)HFD+water extract of Coix 100 mg/kg (10)HFD+phentermine 5 mg/kg (10) 16 weeks Body weight, food intake, energy efficiency, adipocyte size, morphological change (epididymal tissue, liver tissue), liver weight, GOT, GPT, OGTT, serum insulin
Obesity (HFD induced)
11 Kim SM 63) (2016) Efficacy pharmacology; lipid metabolism SD rat (-) Scutellariae Radix (SRE)Oral administration Control-negative (10)SRE 100 mg/kg (10)SRE 200 mg/kg (10)SRE 300 mg/kg (10) 6 weeks FFA, TG, TC, HDL, LDL, GOT, GPT, TBARS (plasma, lier), GSH-px, SOD, CAT, IL-1β, IL-6, TNF-α, IL-10
Abnormal lipid metabolism (induced by high oxidized fat in food stuffs)
12 Kang AN 64) (2016) Efficacy pharmacology; glucose metabolism SD rat (5 weeks) Spagranii Rhizoma (SR)Oral administration Normal (-)Control-negative (-)SR 500 mg/kg (-) 4 weeks BW, food intake, water intake, urine volume, TC, GOT, GPT, histopathologic changes (pancrease, liver, kidney)
Diabetes (high fat, high sucrose diet and STZ administration induced)
13 Chang SJ 65) (2017) Efficacy pharmacology; glucose metabolism C57BL/6 mice (4 weeks) Scutellaria bacalensis+metforminOral administration Normal (7)Control-negative (7)HFD+metformin 100 mg/kg/day (7)HFD+metformin 50 mg/kg/day+Scutellariae Radix 200 mg/kg/day (7)HFD+Scutellariae Radix 400 mg/kg/day (7) 10 weeks BW, OGTT, TC, organ weight (liver, kidney, testis) fat weight (intestinal fat, total fat) GOT, GPT, histological changes of liver tissue
Obesity (HFD induced)
14 Song MY 66) (2017) Efficacy pharmacology; glucose metabolism C57BL/6 mice (5 weeks) Aconitum carmichaeli Debx (ACD)Oral administration Normal (3)HFD (3)HFD+ACD 100 mg/kg (3)HFD+ACD 300 mg/kg (3) 16 weeks BW, food intake, tissue weight (liver, pancreas, MW/BW), adipose tissue weight, AST, ALT, lipid accumuliation in liver, TG, TC, HDL, OGTT, body temperature
Obesity (HFD induced)
15 Kim MJ 67) (2017) Single dose toxicity study SD rat (7 weeks) Modified Samjung-hwan Oral administration Female 0 mg/kg (5)Female 5,000 mg/kg (5)Male 0 mg/kg (5)Male 5,000 mg/kg (5) Once BW, clinical signs, necropsy findings
-
16 Bae JS 68) (2017) Efficacy pharmacology; GI mobility ICR mice (8 weeks) Socheongryong-tang Oral administration Normal (-)Control-negative (-) Poncirus trifoliata Raf. (-) Socheongryong-tang 0.0025 g/kg (-) Socheongryong-tang 0.025 g/kg (-) Socheongryong-tang 0.25 g/kg (-) Once ITR
Peritoneal irritation (acetic acid injection induced)Diabetes (STZ administration induced)
17 Song MY 69) (2017) Efficacy pharmacology; glucose metabolism C57BL/6 mice (5 weeks) CAPA(Cinnamomum cassia Blume, Atractylodes macrocephala Koidzumi, Pueraria lobata Benth, Aconitum carmichaeli Debx)Oral administration Normal (5)HFD (5)HFD+CAPA 100 mg/kg (5)HFD+CAPA 300 mg/kg (5)HFD+metformin 200 mg/kg (5)HFD+lorcaserin 10 mg/kg (5) 16 weeks BW, food intake, energy efficiency, body temperature, tissue weight (liver, pancreas, MW/BW), adipose tissue weight, AST, ALT, lipid accumuliation in liver, TG, TC, HDL, LDL, OGTT,
Obesity (HFD induced)
18 Baek SE 70) (2017) Efficacy pharmacology; extrogen-deficiency Rat(5 weeks) Pueraria lobata (PL)Oral administration Normal - sham operation group (6)Control - ovariectomy group (8)Ovariectomy＋50 mg/kg of PL (8)Ovariectomy＋100 mg/kg of PL (8)Ovariectomy＋200 mg/kg of PL (8) 8 weeks BW, leptin, estradiol, CCK, ghrelin, adiponectin
Estrogen-deficient obesity (Ovariectomized)
19 Im UJ 71) (2017) Efficacy pharmacology; cytoprotective effect SD rat (12 weeks) Salvianolic acid B (SAB)Oral administration Normal (12)Control-negative (12)SAB (12) 5 days AST, LDH, CPK, creatinine, histological changes of left ventricle myocardium, sporadic fragmentation, superoxide formation, expression of Bax, expression of Bcl-2
Cardiac muscle damage (exhaustive swimming induced)
20 Lee YK 72) (2017) Efficacy pharmacology; lipid metabolism C57BL/6 mice (5 weeks) Eriobotrya folium (EFE)Injection. 3 times/week Control saline 100 mLEFE 20 mg/mL, 100 mL 2 weeks BW, histological changes of inguinal body fat pad, HSL, ATGL, lipid accumluation, lipase
Obesity (HFD induced)
21 Cho EJ 73) (2018) Efficacy pharmacology; anti-obesity, lipid metabolism SD rat (6 weeks) Daecheongryoung-tang (DCR)Oral administration Normal (7)Control-negative (9)DCR 250 mg/kg/day (9)DCR 5,000 mg/kg/day (9) 6 weeks BW, TC, TG, HDL, LDL, FFA, total lipid, phospholipid, WBC, RBC, Hb, PLT, GPT, GOT
Obesity (HFD induced)
22 Kim MJ 74) (2018) Repeated dose toxicity study SD rat(5-6 weeks) Modified Samjung-hwan Oral administration Modified Samjung-hwan 0 mg/kg/day (10)Modified Samjung-hwan 1,000 mg/kg/day (10)Modified Samjung-hwan 2,000 mg/kg/day (10)Modified Samjung-hwan 4,000 mg/kg/day (10) 90 days Clinical sign, mortality, food intake, BW, ophthalmologic findings, urine analysis, hematology, serum biochemistry, necropsy findings, organ weights, histological markers
-
23 Kim BH 75) (2018) Efficacy pharmacology; lipid metabolism SD rat (8 weeks) Scutellaria Radix Oral administration Normal (10)Control-negative (10)EtOH+Scutellaria Radix (10) 8 weeks Mortality, clinical sign, BW, AST, ALT, liver weight, liver-body weight, histological chagnes in liver tissue, TNF-α
Alcoholic fatty liver (induced by long-term EtOH administration)
24 Kim BH 76) (2018) Efficacy pharmacology; cytoprotective effect SD rat (8 weeks) Silbi-um Oral administration Normal (10)Control-negative (10)EtOH+Silbi-um (10) 8 weeks BW, AST, ALT, AST/ALT, liver weight, liver-body weight, histological chagnes in liver tissue, TNF-α
Alcoholic fatty liver (induced by EtOH)

HCT: hematocrit, LPS: lipopolysaccharide, TEER: transepithelial electrical resistance, NO: nitric oxide, HRP: horseradish peroxide, DPPH: 2,2-diphenyl-1-picrylhydrazyl, COX-2: cyclooxygenase-type 2, TEER: transepithelial electrical resistance, C/EBPα: CCAAT/enhancer-binding protein α, PPARγ: peroxisome proliferators-activated receptor γ, AMPK: adenosine monophosphate-activated kinase, PPAR-α: peroxisome proliferators-activated receptor α, IL: interleukin, TNF: tumor necrosis factor, XBP-1: X-box binding protein 1, SIRT 1: sirtuin1, PGC1α: peroxisome proliferators-activated receptror γ coactivator 1α, NRF: nuclear respiratory factors, Tfam: mitochondrial transcription factor, HMG-CoA: 3-hydroxy-3-methylglutaryl CoA reducatase, MyoD: myoblast determination protein1, Mrf5: myogenic factor 5, MHC: myosin heavy chain, GAPDH: glyceraldehyde-3-phosphate dehydrogenase, ROS: reactive oxygen species, Bcl-2: B-cell lymphoma 2, PARP: poly [ADP-ribose]-polymerase, HO-1: heme oxygenase 1, NQO-1: NAD(P)H dehydrogenase quinone 1, TrxR1: thioredoxin reductase 1, ATP: adenosine triphosphate, JNK: Jun N-terminal kinase, NF-κB: nuclear factor-κB, ACC: acetyl-CoA carboxylase, NAO: nonyl acridine orange, TRPM7: transient receptor potential melastatin 7, IC: the half maximal inhibitory concentration, DNJ: 1-deoxynojirimycin, GABA: γ-aminobutyric acid, ACE: angiotensinconverting-enzyme, 2-NBDG: 2-[N-(7-160 nitrobenz-2-oxa-1,3,-diazol-4-yl)amino]-2-deoxy-dglucose, FFA: free fatty acid, TRPV4: transient receptor potential melastatin 7, NAC: N-acetyl cysteine, MMPs: mitochondrial membrane potentials, Bcl-2: B-cell lymphoma 2, 2-DG6P: 2-deoxyglucose-6-phosphate, IRS-1: insulin receptor substrate-1, Akt: protein kinase B, GLUT4: glucose transporter 4.
J Korean Med Obes Res 2020;20:149-77 https://doi.org/10.15429/jkomor.2020.20.2.149
© 2020 J Korean Med Obes Res