Fig. 6. Effect of tanshinone I on lipid accumulation in zebrafish. Two dpf larvae were exposed in the absence (DMSO) or presence of tanshinone I at the indicated concentrations. After 24 h, 3 dpf larvae were stained with LipidGreen2 and visualized under bright field (A) and fluorescence field (B). (C, D) Size and intensity of yolk (white dash in B) were quantified using the ImageJ software (U.S. National Institutes of Health, Bethesda, MD, USA), respectively. DMSO: dimethyl sulfoxide, Tan I: tanshinone I. Graph bars indicate mean±standard error of the mean. Unpaired t-test was used to analyze difference versus DMSO group, and statistical significance was set 0.05 and 0.01 (*P≤0.05, **P≤0.01). n=10.
