Primer Sequences Used for RT-PCR
| Gene | Sequence | |
|---|---|---|
| C/EBP-α | Sense | 5′-TTACAACAGGCCAGGTTTCC-3′ |
| Antisense | 5′-CTCTGGGATGGATCGATTGT-3′ | |
| PPAR-γ | Sense | 5′-GGTGAAACTCTGGGAGATTC-3′ |
| Antisense | 5′-CAACCATTGGGTCAGCTCTC-3′ | |
| FAS | Sense | 5′-TTGCTGGCACTACAGAATGC-3′ |
| Antisense | 5′-AACAGCCTCAGAGCGACAAT-3′ | |
| Perilipin A | Sense | 5′-CTTTCTCGACACACCATGGAAACC-3′ |
| Antisense | 5′-CCACGTTATCCGTAACACCCTTCA-3′ | |
| Leptin | Sense | 5′-CCAAAACCCTCATCAAGACC-3′ |
| Antisense | 5′-CTCAAAGCCACCACCTCTGT-3′ | |
| ACC | Sense | 5′-GTCAGCGGATGGGCGGAATG-3′ |
| Antisense | 5′-CGCCGGATGCCATGCTCAAC-3′ | |
| Actin | Sense | 5′-TCATGAAGTGTGACGTTGACATCCGT-3′ |
| Antisense | 5′-CCTAGAAGCATTTGCGGTGCACGATG-3′ |
RT-PCR: reverse transcription polymerase chain reaction, C/EBP-α: CCAAT/enhancer-binding protein-α, PPAR-γ: peroxisome proliferator-activated receptor-γ, FAS: fatty acid synthase, ACC: acetyl CoA carboxylase.
