HPLC Analytical Conditions
| Classification | Condition | Explanation | ||
|---|---|---|---|---|
| 1st condition | Instrument | Agilent 1260 series | ||
| Column | Phenyl-Hexyl column | |||
| Oven temperature | 25℃ | |||
| Wave length | UV 254 nm | |||
| Injection volume | 20 μL | |||
| Mobile phase | A: 0.1% Trifluoroacetic acid in H2O | |||
| B: Acetonitrile | ||||
| Time (min) | A (%) | B (%) | ||
| 0 | 92 | 8 | ||
| 15 | 92 | 8 | ||
| 20 | 90 | 10 | ||
| 25 | 90 | 10 | ||
| 80 | 53 | 47 | ||
| 85 | 40 | 60 | ||
| 90 | 40 | 60 | ||
| 2nd condition | Instrument | Agilent 1260 series | ||
| Column | YMC-Triat C18 (5 μm, 4.6×250 mm) | |||
| Oven temperature | 25℃ | |||
| Wave length | UV 210 nm | |||
| Injection volume | 20 μL | |||
| Mobile phase | A : 0.1% H3PO4 in H2O | |||
| B : Acetonitrile | ||||
| Time (min) | A (%) | B (%) | ||
| 0 | 95 | 5 | ||
| 35 | 95 | 5 | ||
| 40 | 85 | 15 | ||
| 50 | 85 | 15 | ||
| 70 | 55 | 45 | ||
| 75 | 0 | 100 | ||
| 90 | 0 | 100 | ||
